Acyl chain orientational order in large unilamellar vesicles: comparison with multilamellar liposomes: a 2H and 31P nuclear magnetic resonance study
نویسندگان
چکیده
منابع مشابه
Acyl chain orientational order in large unilamellar vesicles: comparison with multilamellar liposomes: a 2H and 31P nuclear magnetic resonance study.
Large unilamellar vesicles (LUVs) composed of 1-[2H31]palmitoyl-2-oleoyl phosphatidylcholine (POPC-d31), with diameters of approximately 117 +/- 31 and 180 +/- 44 nm, were prepared by extrusion through polycarbonate filters with pore sizes of 0.1 and 0.2 microns, respectively. The 2H nuclear magnetic resonance (NMR) spectra obtained at 21 degrees C contain two components: a broad component (app...
متن کاملAcyl Chain Orientational Order
The deuterium nuclear magnetic resonance (2H NMR) spectrum of perdeuterated tetradecanol in a mixture of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) and water was used to compare the variation of the acyl chain orientational order parameter, S(n), with carbon position, n, in the liquid crystalline lamellar (La) and hexagonal (HI,) phases. The characteristic independence of S(n) with n ...
متن کامل19F-nuclear magnetic resonance study of glycerolipid fatty acyl chain order in Acholeplasma laidlawii B membranes.
The technique of 19F-nuclear magnetic resonance (19F-NMR) spectroscopy offers a number of advantages for studies of lipid fatty acyl chain orientation and dynamics in biomembranes. However, the geminal difluoromethylene fatty acid probes usually employed in such studies appreciably perturb the organization of lipid bilayers. We have thus synthesized a series of specifically monofluorinated palm...
متن کامل31P-nuclear magnetic resonance spectroscopy of blood: a species comparison.
31P-nuclear magnetic resonance (NMR) spectroscopy isfrequently used as a tool in the study of organs from various animal species and humans. Because signals arising from the presence of blood are visible in in vivo 31P-NMR spectra of blood-filled organs, such as the heart, it is necessary to correct these spectra for the contribution of blood to the signal. It is unknown whether species differe...
متن کامل[2H]Hydrogen and [31P]phosphorus nuclear-magnetic-resonance and neutron-diffraction studies of membranes.
Margaritis, L. H., Elgsaeter, A. & Branton, D. (1977) J . Cell Biol. 72, 47-56 Moor, H. (1966) Int. Rev. Exp. Path. 5 , 179-216 Moor, H. (1971) Phil. Trans. R . SOC. London Ser. B261,121-131 Nicolson, G. L. (1974) Int. Rev. Cytol. 39, 89-190 Pfenninger, K. H. & Rinderer. E. R. (1 975) J. Cell Biol. 65, 15-28 Pinto da Silva, P. & Branton, D. (1970) J. Cell Bio/. 45,598-605 Pinto da Silva, P. & B...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1993
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(93)81515-7